回複 '雅美之途' 的評論 : PCR得的是化學獎,不是生理學&醫學獎; 單克隆抗體是克隆選擇學說最直接的證明,理論意義極其重大,所以當年的獲獎者中包括了Jerne。some procedures for medicine,比如骨髓移植是因為直接拯救了生命,跟青蒿素一樣,側重於生理及醫學中的醫。你列出的https://www.nobelprize.org/nobel_prizes/medicine/laureates/2007/advanced.html不過是對Gene targeting 做知識介紹,cite了幾十篇文獻,包括與得獎者毫無關係的Cre-Loxp係統。即便這樣,87年的HPRT paper 顯然是重點介紹,而不是85年的globin。
雅美之途 發表評論於
回複 'czhz' 的評論 : One citation was from Nobel committee. Please don't forget Nobel Prizes for PCR, monoclonal antibody and some procedures for medicine.
czhz 發表評論於
當年的諾獎也是與ES Cell 綁在一起發的。
czhz 發表評論於
回複 '雅美之途' 的評論 : Smithies 當然推自己85年的文章,而不是87年的文章,因為這樣就早2年,也比Marrio 的文章早。To the best of knowledge, Gene Targeting 是唯一因技術而獲諾貝爾生理醫學獎的成果,其它都是因揭示生命現象和理論而得獎。而Gene Targeting 作為技術是與ES Cell連在一起的才得以應用,85年的文章沒有用到ES Cell, 不具實用性,87年Tom的Nature文章才用到了ES Cell,才能做knock out 老鼠。
雅美之途 發表評論於
回複 'czhz' 的評論 : You completely messed up.
"The strategy was successful and he reported in a landmark paper in the September 19, 1985 issue of Nature the successful integration by homologous recombination of a plasmid into the chromosomal β-globin gene of human erythroleukaemia cells [24].
[24] Smithies O, Gregg, R. G., Boggs, S. S., Doralewski, M. A., Kucherlapati, R. S. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature. 1985;317:230-4.
回複 'czhz' 的評論 : You are simply wrong. Please see here from Smithies' biography:
"But without this decision I might not have had the time to start the experiments, begun at age 57, which led to my best gene targeting paper, published after I was 60 (Smithies et al., 1985)."
He won Nobel virtually by this paper published in Nature:
Smithies, O., Gregg, R. G., Boggs, S. S., Koralewski, M. A., and Kucherlapati, R. S. (1985). Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature 317, 230-234.