圖５裏有兩個predicted. 我不是很清楚閻潤濤說的是哪個， 就兩個都談談我的理解。
一： Shown here are the predicted conformations—outward-open, ligand-bound and occluded, inward-open, and ligand-free and occluded—required for a complete transport cycle according to the alternating access model.
粗體的部分我不是很清楚是應該理解為：
（１）Shown here are the conformations predicted by the scientific community. 還是
（2） Shown here are the conformation predicted through our experiments.
如果是（1）那麽沒問題。如果是（2）需要先解釋第二個predict.
二。 The ligand-bound, occluded conformation and the ligand-free, occluded one are predicted from two XylE structures in the outward-facing, partly occluded and ligand-bound state
Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); ...If. condition ... (then) one might also view this transporter as a "GATED PORE"
“when the transporter is in its outward-facing conformation, external substrates (or probes) would contact domains C and B but not domain A. Conversely, when in the inward-facing conformation, substrates (or probes) could interact with domains A and B but not with domain C. Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); substrate transport arises as the formation and collapse of barriers (domains A and C) forces the translocation pathway to communicate with the inner and outer aqueous phases in alternating sequence. If the core domain B functions solely as a passive conduit for substrate movement, one might also view this transporter as a "GATED PORE" but before adopting this terminology it may be best to await a better experimental definition of this central region.”
注22. Mitchell, P. (1990) Res. Microbiol. 141, 286-289
“when the transporter is in its outward-facing conformation, external substrates (or probes) would contact domains C and B but not domain A. Conversely, when in the inward-facing conformation, substrates (or probes) could interact with domains A and B but not with domain C. Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); substrate transport arises as the formation and collapse of barriers (domains A and C) forces the translocation pathway to communicate with the inner and outer aqueous phases in alternating sequence. If the core domain B functions solely as a passive conduit for substrate movement, one might also view this transporter as a "GATED PORE" but before adopting this terminology it may be best to await a better experimental definition of this central region.”
注22. Mitchell, P. (1990) Res. Microbiol. 141, 286-289
就因為在7月15日的帖子“轉運蛋白模型一日遊 （五）總結”的最初版本中，我和你討論，你說“目前看過的文獻來看， Yan_Maloney模型隻出現在閻的博客裏，沒有出現在科學雜誌裏，就是說在科學界不存在”。我隻好問你“‘The best characterized members of the family are UhpT and GlpT, both of E. coli, for which detailed topological models have been presented (29, 90, 91).’ 這是被引用了1281次的綜述“Major facilitator superfamily（1998）”文章裏的原話。其中文獻90， 91分別是大閻1993和1995的文章。隊友閣下，在這裏"models"是啥意思？”。我再說“這就是你‘看了這麽多文獻，沒有一篇文獻用模型一詞來提閻的兩篇論文’。反證”，到這時你才無奈地承認你錯了，有大閻的模型。
