“The purified recombinant SARS-CoV-2 S1 protein was mixed and emulsified with Freund's immune adjuvant in equal volume and then used as an immunogen. Each hen was injected (intramuscular) with 150 μg of the recombinant spike protein under the wings, once a week for 4 weeks, and then IgY was extracted and the titer evaluated. ”