人在做天在看，天網恢恢，疏而不漏。武漢病毒中國製造，最終讓中國人享用，作惡就要受懲罰。真正的教科書級別的搬起石頭砸自己的腳！
Nature Medicine (2015, GOF research)
A SARS-like cluster of circulating bat coronaviruses
shows potential for human emergence
Vineet D Menachery1, Boyd L Yount Jr1, Kari Debbink1,2, Sudhakar Agnihothram3, Lisa E Gralinski1,
Jessica A Plante1, Rachel L Graham1, Trevor Scobey1, Xing-Yi Ge4, Eric F Donaldson1, Scott H Randell5,6,
Antonio Lanzavecchia7, Wayne A Marasco8,9, Zhengli-Li Shi4 & Ralph S Baric1,2
Abstruct:
Using the SARS-CoV reverse genetics system2,
we generated and characterized a chimeric virus expressing
the spike of bat coronavirus SHC014 in a mouse-adapted
SARS-CoV backbone. The results indicate that group 2b
viruses encoding the SHC014 spike in a wild-type backbone
can efficiently use multiple orthologs of the SARS receptor
human angiotensin converting enzyme II (ACE2), replicate
efficiently in primary human airway cells and achieve in vitro
titers equivalent to epidemic strains of SARS-CoV. Additionally,
in vivo experiments demonstrate replication of the chimeric
virus in mouse lung with notable pathogenesis.
On the basis of these findings, we synthetically re-derived an infectious
full-length SHC014 recombinant virus and demonstrate robust
viral replication both in vitro and in vivo. Our work suggests a
potential risk of SARS-CoV re-emergence from viruses currently
circulating in bat population。
