A story from my personal experience about reference letters
My 1-140 got approved a few months ago after RFE. In my initial submission, I ted 10 reference letters (5 independence and 5 none -independence). The RFE letter from the IO stated that I need to 1-2 more letters from independent sources(persons that I have never worked for or stayed with) showing that my previous contributions are outstanding and can justify my application. In the letter the IO also emphasized that "Attestations from current and prior supervisors tend also to support a conclusion that the national interest would NOT be adversely affected if a employer were to request permanent employment certification from the Department of Labor, and to file a immigrant petition to what would then be the alien beneficiary/petitioner’s behalf". From here I understood that the letters from my current and prior supervisors did not help me at all! My application got approved 3 days after they received my RFE.
My experience told me that letters from bosses are not helpful in such applications.