PNAS2003年關於GEOSMIN合成基因的文章,引發了2004年強哥及自己的一些想法。 1,探求藍藻中相關的基因(強哥) 2,基因敲除---合成途經(我) 3,FPPS/GPPS基因---引物合成---特異專一條帶的得到----部分序列----適用於至少2種以上的產MIB,GEOSMIN的藍藻(我,2004) +++++++++ 4,基因walker kit的發現,產生用walker法得到FPPS/GPPS基因全長及相鄰序列的想法(05/2005) 5,檢查Streptomyces coelicolor genome,發現SCO6073 (cyclase)與FPPS/GPPS/。。並不是在相近的區, Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N
SCD20.01 SCO4583 putative transferase 5004916:5005926 for... 241 9.0e-25 2 2SC7G11.12 gtr SCO5250 polyprenyl synthetase 5711116:5712... 242 6.1e-22 1 crtB SCO0185 putative geranylgeranyl pyrophosphate syntha... 198 3.1e-15 1 SC6A5.12 SCO6763 putative polyprenyl synthatase 7519463:7... 190 2.6e-14 1 SCF73.12c SCO0565 putative polyprenyl synthetase 607851:6... 171 3.8e-12 1 SC8B3.02c SCO0568 putative polyprenyl synthetase 610415:6... 159 9.8e-11 1
Only 6 polyprenyl synthetase-like proteins were detected in its genome. In 6803 genome, similar position were found,sll0506 | CyanoBase | undecaprenyl pyrophosphate synthetase | PEDANT | gi_1001342
COG0020 Undecaprenyl pyrophosphate synthase 1e-111
| slr0611 | CyanoBase | solanesyl diphosphate synthase (sds) | PEDANT | gi_3426161
PIR:S76966 geranylgeranyl pyrophosphate synthase crtE homolog - Synechocystis sp. (strain PCC 6803) 3e-28
COG0142 Geranylgeranyl pyrophosphate synthase 2e-17
| slr0739 | CyanoBase | geranylgeranyl pyrophosphate synthase (crtE) | CYORF | geranylgeranyl pyrophosphate synthase (crtE) | PEDANT | gi_1651762
PIR:S74538 geranylgeranyl pyrophosphate synthase - Synechocystis sp. (strain PCC 6803) 1e-153
COG0142 Geranylgeranyl pyrophosphate synthase 1e-108 |
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